Highlights
- •We provide the first study attempting to understand colonic pH, bicarbonate, and motility profiles from patients with cystic fibrosis.
- •Colonic pH and bicarbonate concentration is impaired in PwCF, particularly in the distal rectosigmoid.
- •These changes likely originate from alterations in intestinal ion transport rather than colonic dysmotility.
- •SLC26A3 is abundantly expressed in the human colon and rectum and may be a potential therapeutic target for restoration of bicarbonate transport in PwCF.
- •Our findings may help better understand the gastrointestinal symptoms in PwCF and provides the framework for future studies in this area.
Abstract
Previous wireless motility capsule (WMC) studies demonstrated decreased small intestinal
pH in people with CF (PwCF) however the data is lacking on the colonic pH profile.
We re-analyzed previously published WMC data to determine colonic pH/bicarbonate concentration
and single cell RNA sequencing (sc-RNAseq) to examine the normal expression of acid-base
transporters in the colon/rectum.CF patients showed significantly lower pH and bicarbonate
concentration values, particularly in the distal rectosigmoid region. There was no
difference in colonic motility parameters between CF and non-CF subjects. SLC26A3
is highly expressed bicarbonate transporter in the colon and rectum, more so than
CFTR. While dysmotility can alter intraluminal pH, observed changes likely originate
from alterations in intestinal ion transport rather than colonic dysmotility. SLC26A3
is abundantly expressed in the human colon and rectum and may be a therapeutic target
for restoration of bicarbonate transport. These findings may help better understand
the gastrointestinal symptoms in PwCF
Keywords
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Article info
Publication history
Published online: December 24, 2022
Accepted:
December 20,
2022
Received in revised form:
September 27,
2022
Received:
August 18,
2022
Publication stage
In Press Corrected ProofIdentification
Copyright
© 2022 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
