Abstract
This article describes the techniques of isolation and culture of human airway epithelial
cells from large airways and from distal airways. Both cell types are obtained from
lung pieces collected during surgery. The protocols start with an initial step of
washing and dissection of the lung pieces to separate large airways or distal airways
from the surrounding parenchyma. The second step is enzymatic isolation of epithelial
cells from the dissected large or distal airways. Cells are then collected by centrifugation
and then seeded onto collagen surfaces. Epithelial cells can be grown at an air–liquid
interface and usually form a confluent and functional epithelial layer within days.
Keywords
References
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© 2004 European Cystic Fibrosis Society. Published by Elsevier Inc.
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