Journal of Cystic Fibrosis
Volume 9, Issue 4 , Pages 277-279, July 2010

Effect of Th2 type cytokines on hCLCA1 and mucus expression in cystic fibrosis airways

  • Hans-Peter Hauber

      Affiliations

    • Division of Pathophysiology of Inflammation, Research Center Borstel, Borstel, Germany
    • Meakins-Christie Laboratories, McGill University, Montreal, Canada
    • Corresponding Author InformationCorresponding author. Division of Pathophysiology of Inflammation, Research Center Borstel, Parkallee 35, 23845 Borstel, Germany. Tel.: +49 4537 188 364; fax: +49 4573 188 313.
    • ,
  • Francois Lavigne

      Affiliations

    • Meakins-Christie Laboratories, McGill University, Montreal, Canada
    • ,
  • Hsiao-Ling Hung

      Affiliations

    • Genaera Corporation, Plymouth Meeting, PA, USA
    • ,
  • Roy C. Levitt

      Affiliations

    • Genaera Corporation, Plymouth Meeting, PA, USA
    • ,
  • Qutayba Hamid

      Affiliations

    • Meakins-Christie Laboratories, McGill University, Montreal, Canada

Received 28 March 2010; received in revised form 3 May 2010; accepted 5 May 2010.

Abstract 

Correlations between expression of interleukin (IL)-9, the calcium-activated chloride channel hCLCA1 and mucus expression in cystic fibrosis (CF) airways have suggested a causal relationship. To verify this hypothesis mucosal tissue from upper airways of CF patients (N=5) was stimulated with the Th2 type cytokines IL-4, IL-9, or IL-13. Expression of hCLCA1 mRNA and protein as well as mucus and mucin (MUC5AC) gene expression was quantified using real time PCR, immunohistochemistry (hCLCA1) and PAS staining (mucus). Th2 type cytokines significantly increased hCLCA1 protein expression (P<0.05) whereas increase in hCLCA1 mRNA expression failed to reach statistical significance (P>0.05). Mucin protein and MUC5AC mRNA expression were not significantly changed (P>0.05). These data suggest that Th2 type cytokines may increase hCLCA1 expression in CF but may not have a significant effect on mucus expression. Therefore the role of hCLCA1 as a mediator of mucus overexpression in CF has to be questioned.

Keywords: Airway, Cystic fibrosis, hCLCA1, Mucosa, Mucus, Th2 type cytokine

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PII: S1569-1993(10)00067-6

doi:10.1016/j.jcf.2010.05.002

Journal of Cystic Fibrosis
Volume 9, Issue 4 , Pages 277-279, July 2010

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